Hong-Bin Shen

A single-cell transcriptome snapshot carries both where a cell sits along a trajectory and which way its expression is moving, and these were usually estimated separately. ManifoldVelo places each cell on a differentiable unspliced/spliced RNA expression curve and matches the curve's derivatives to a transcription–splicing–degradation kinetic field, including between observed cells, so coordinates, curves and kinetics are fitted together. In multipeak cyclic simulations, spliced-only queries gave circular time errors of 0.64% and 7.40% with spliced-velocity cosines of 0.9776 and 0.9421; on WT2, phase error fell 47.0% relative to the recorded VeloCycle result, while VeloCycle did better on WT1 and the smooth cycle.

A single-cell transcriptome snapshot carries both where a cell sits along a trajectory and which way its expression is moving, and these were usually estimated separately. ManifoldVelo places each cell on a differentiable unspliced/spliced RNA expression curve and matches the curve's derivatives to a transcription–splicing–degradation kinetic field, including between observed cells, so coordinates, curves and kinetics are fitted together. In multipeak cyclic simulations, spliced-only queries gave circular time errors of 0.64% and 7.40% with spliced-velocity cosines of 0.9776 and 0.9421; on WT2, phase error fell 47.0% relative to the recorded VeloCycle result, while VeloCycle did better on WT1 and the smooth cycle.