Combining limma differential analysis, WGCNA, a GeneCards oxidative-stress gene set, PPI networks, and three machine learning methods (LASSO, random forest, SVM-RFE), the study narrowed obstructive sleep apnea (OSA) adipose transcriptomes to 57 shared differentially expressed genes and two hub genes, JAK2 and ANXA5, then used single-cell sequencing, scTenifoldKnk virtual knockout, immune deconvolution, RT-qPCR, and Western blotting to show that JAK2 is significantly upregulated and ANXA5 significantly downregulated in OSA, that both are enriched in monocytes, and that CPAP treatment lowers JAK2 while raising ANXA5.
Combining limma differential analysis, WGCNA, a GeneCards oxidative-stress gene set, PPI networks, and three machine learning methods (LASSO, random forest, SVM-RFE), the study narrowed obstructive sleep apnea (OSA) adipose transcriptomes to 57 shared differentially expressed genes and two hub genes, JAK2 and ANXA5, then used single-cell sequencing, scTenifoldKnk virtual knockout, immune deconvolution, RT-qPCR, and Western blotting to show that JAK2 is significantly upregulated and ANXA5 significantly downregulated in OSA, that both are enriched in monocytes, and that CPAP treatment lowers JAK2 while raising ANXA5.