The Netherlands Cancer Institute

The study presents HARP (Human Cell Atlas Reference for PBMCs), an integrated atlas of ~9 million peripheral blood mononuclear cells (PBMCs) from >2,600 donors across 15 studies spanning four continents, neonates to 97 years, and health and diverse immune-related diseases; it develops optimized integration workflows with novel label-free metrics for integration quality, generates community-driven consensus annotations for 192 immune cell subsets including rare populations as low as 0.

The study presents HARP (Human Cell Atlas Reference for PBMCs), an integrated atlas of ~9 million peripheral blood mononuclear cells (PBMCs) from >2,600 donors across 15 studies spanning four continents, neonates to 97 years, and health and diverse immune-related diseases; it develops optimized integration workflows with novel label-free metrics for integration quality, generates community-driven consensus annotations for 192 immune cell subsets including rare populations as low as 0.

This work presents AlphaBridge, a reproducible, objective, and automated toolkit, also available as a web server, that combines AlphaFold3 confidence metrics to cluster sequence motifs participating in binary interactions and subsequently in 3D interfaces of complexes, visualizes interaction interfaces within confidence limits via chord diagrams, network graphs, and summary tables of predicted interfaces and intermolecular interactions linked to interactive graphics, and was validated for scoring binary and multi-component protein complexes with real-life examples discussed.

This work presents AlphaBridge, a reproducible, objective, and automated toolkit, also available as a web server, that combines AlphaFold3 confidence metrics to cluster sequence motifs participating in binary interactions and subsequently in 3D interfaces of complexes, visualizes interaction interfaces within confidence limits via chord diagrams, network graphs, and summary tables of predicted interfaces and intermolecular interactions linked to interactive graphics, and was validated for scoring binary and multi-component protein complexes with real-life examples discussed.