# Adenosine-to-inosine RNA editing

Locating RNA inosine sites has long relied on A-to-G base transitions in sequencing, which are hard to separate from DNA mutations and sequencing errors. REX-seq couples EndoV cleavage at inosines with XRN-1 degradation of fragments bearing 5′ phosphate ends, yielding cleavage signatures while preserving gene-expression quantification; in HEK293T cells it identified 590,249 high-confidence inosine sites, 84,645 of them not previously reported. Trained on these sites, InoDREAM reached an AUROC of 0.965 and average precision of 0.964 on a held-out test set, against 0.921 and 0.890 for EditPredict.

Locating RNA inosine sites has long relied on A-to-G base transitions in sequencing, which are hard to separate from DNA mutations and sequencing errors. REX-seq couples EndoV cleavage at inosines with XRN-1 degradation of fragments bearing 5′ phosphate ends, yielding cleavage signatures while preserving gene-expression quantification; in HEK293T cells it identified 590,249 high-confidence inosine sites, 84,645 of them not previously reported. Trained on these sites, InoDREAM reached an AUROC of 0.965 and average precision of 0.964 on a held-out test set, against 0.921 and 0.890 for EditPredict.