# bioinformatics pipeline

This study devised a bioinformatics pipeline for a comparative analysis of mechanosensitive membrane proteins in lactic acid bacteria (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and a fungus (Pleurotus floridanus), generating a consensus sequence for P. floridanus via multiple sequence alignment of homologues and mapping it onto the genome with BLAST to delineate the gene region of interest, then producing protein structures with the AlphaFold3 artificial intelligence platform using the correct oligomeric state for each channel, thereby bridging the structural knowledge gap for mechanoreceptors in these non-model systems.

This study devised a bioinformatics pipeline for a comparative analysis of mechanosensitive membrane proteins in lactic acid bacteria (Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus) and a fungus (Pleurotus floridanus), generating a consensus sequence for P. floridanus via multiple sequence alignment of homologues and mapping it onto the genome with BLAST to delineate the gene region of interest, then producing protein structures with the AlphaFold3 artificial intelligence platform using the correct oligomeric state for each channel, thereby bridging the structural knowledge gap for mechanoreceptors in these non-model systems.