Hitesh Bhagavanbhai Mangukiya

Glioblastoma cells switch among branching, diffuse translocation, locomotion and perivascular invasion modes, but these switches were hard to observe at scale over days in intact brain tissue. GlioTrace links whole-specimen confocal imaging, convolutional-network morphology classification and a covariate hidden Markov model to track thousands of cells across six patient-derived xenograft brain slices. Invasion plasticity proved patient-specific and spatially organized, modulated by vascular proximity and microglial contact; targeted drugs shifted phenotype composition and transition probabilities, while radiation plus temozolomide enriched the fastest, highly connected branching state.

Glioblastoma cells switch among branching, diffuse translocation, locomotion and perivascular invasion modes, but these switches were hard to observe at scale over days in intact brain tissue. GlioTrace links whole-specimen confocal imaging, convolutional-network morphology classification and a covariate hidden Markov model to track thousands of cells across six patient-derived xenograft brain slices. Invasion plasticity proved patient-specific and spatially organized, modulated by vascular proximity and microglial contact; targeted drugs shifted phenotype composition and transition probabilities, while radiation plus temozolomide enriched the fastest, highly connected branching state.