Shiji Liu

Single-cell 3D genome data are so sparse that domain boundaries are hard to call reliably, and positional disagreement across cells blocks comparison of boundary frequency and strength. CellRoB couples single-cell boundary detection with cross-cell alignment that minimizes an insulation-score loss, improving boundary accuracy and robustness to contact dropout on mouse brain Dip-C and human GM12878 scMicro-C data from 40 kb down to 5 kb. On multimodal mouse embryo data, boundary-strength profiles split the EPI cells that the RNA embedding left as one group.

Single-cell 3D genome data are so sparse that domain boundaries are hard to call reliably, and positional disagreement across cells blocks comparison of boundary frequency and strength. CellRoB couples single-cell boundary detection with cross-cell alignment that minimizes an insulation-score loss, improving boundary accuracy and robustness to contact dropout on mouse brain Dip-C and human GM12878 scMicro-C data from 40 kb down to 5 kb. On multimodal mouse embryo data, boundary-strength profiles split the EPI cells that the RNA embedding left as one group.