Université d'Artois

Once lymph-node tissue is mixed into finely comminuted ground beef, visual inspection can no longer find it, while DNA and protein methods are specific but destructive and need wet-chemistry preparation. Four-excitation front-face fluorescence spectroscopy with an explainable LSTM was trained on 824 samples from five slaughterhouses and three breeds, then tested on two unseen slaughterhouses, reaching R2P 0.988–0.993, RMSEP 2.60–3.35% w/w and RPD 9.3–12.0. An excitation ablation attributes the specificity to multi-excitation sampling rather than the network, with a 1.8% w/w detection limit and a 6.0% w/w quantification limit.

Once lymph-node tissue is mixed into finely comminuted ground beef, visual inspection can no longer find it, while DNA and protein methods are specific but destructive and need wet-chemistry preparation. Four-excitation front-face fluorescence spectroscopy with an explainable LSTM was trained on 824 samples from five slaughterhouses and three breeds, then tested on two unseen slaughterhouses, reaching R2P 0.988–0.993, RMSEP 2.60–3.35% w/w and RPD 9.3–12.0. An excitation ablation attributes the specificity to multi-excitation sampling rather than the network, with a 1.8% w/w detection limit and a 6.0% w/w quantification limit.