Public articles linked to the same research event.
bioRxiv This work introduces an intelligent sequential proximity ligation assay (iseqPLA) read out by spinning disk confocal microscopy and 3D reconstruction to profile endogenous NF-κB protein-protein interactions inside single cells, treating clusters of co-localized puncta as a measure of supercomplex spatial organization, and tracks supercomplex dissociation, p65 nuclear translocation, and negative-feedback engagement across cytokine time courses in NIH-3T3 mouse fibroblasts, cystic fibrosis (CF) patient-derived macrophage co-cultures with IMR-90 human fibroblasts, and an independent set of healthy- and CF-donor monocyte-fibroblast co-cultures, reporting three findings: 3D volumetric quantification reduces the variance in nuclear-to-cytoplasmic ratio measurements relative to 2D projections, th
This work introduces an intelligent sequential proximity ligation assay (iseqPLA) read out by spinning disk confocal microscopy and 3D reconstruction to profile endogenous NF-κB protein-protein interactions inside single cells, treating clusters of co-localized puncta as a measure of supercomplex spatial organization, and tracks supercomplex dissociation, p65 nuclear translocation, and negative-feedback engagement across cytokine time courses in NIH-3T3 mouse fibroblasts, cystic fibrosis (CF) patient-derived macrophage co-cultures with IMR-90 human fibroblasts, and an independent set of healthy- and CF-donor monocyte-fibroblast co-cultures, reporting three findings: 3D volumetric quantification reduces the variance in nuclear-to-cytoplasmic ratio measurements relative to 2D projections, th
This work introduces an intelligent sequential proximity ligation assay (iseqPLA) read out by spinning disk confocal microscopy and 3D reconstruction to profile endogenous NF-κB protein-protein interactions inside single cells, treating clusters of co-localized puncta as a measure of supercomplex spatial organization, and tracks supercomplex dissociation, p65 nuclear translocation, and negative-feedback engagement across cytokine time courses in NIH-3T3 mouse fibroblasts, cystic fibrosis (CF) patient-derived macrophage co-cultures with IMR-90 human fibroblasts, and an independent set of healthy- and CF-donor monocyte-fibroblast co-cultures, reporting three findings: 3D volumetric quantification reduces the variance in nuclear-to-cytoplasmic ratio measurements relative to 2D projections, th
This work introduces an intelligent sequential proximity ligation assay (iseqPLA) read out by spinning disk confocal microscopy and 3D reconstruction to profile endogenous NF-κB protein-protein interactions inside single cells, treating clusters of co-localized puncta as a measure of supercomplex spatial organization, and tracks supercomplex dissociation, p65 nuclear translocation, and negative-feedback engagement across cytokine time courses in NIH-3T3 mouse fibroblasts, cystic fibrosis (CF) patient-derived macrophage co-cultures with IMR-90 human fibroblasts, and an independent set of healthy- and CF-donor monocyte-fibroblast co-cultures, reporting three findings: 3D volumetric quantification reduces the variance in nuclear-to-cytoplasmic ratio measurements relative to 2D projections, th