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bioRxiv (Cold Spring Harbor Laboratory)

HARP integrates ~9 million PBMCs with 192 immune cell annotations and uncovers a sex dimorphism in prostaglandin signaling

The study presents HARP (Human Cell Atlas Reference for PBMCs), an integrated atlas of ~9 million peripheral blood mononuclear cells (PBMCs) from >2,600 donors across 15 studies spanning four continents, neonates to 97 years, and health and diverse immune-related diseases; it develops optimized integration workflows with novel label-free metrics for integration quality, generates community-driven consensus annotations for 192 immune cell subsets including rare populations as low as 0.